The Androlab, Inc (Andrology Laboratory Services Inc.) is an independent fertility lab located in the Streeterville area of Downtown Chicago.
During coitus, the ejaculate is deposited around the cervix. Only progressively motile sperm having a normal morphology can penetrate and migrate through the cervical mucus.
In addition, there are factors in seminal plasma that actually inhibit fertilization (de capacitation factors), and prostaglandin. Although fertile sperm with progressive motility and normal morphology are able to pass through into the uterus, they can’t fertilize an egg until its seminal plasma coating are removed.
The sperm processing performed in the laboratory removes these harmful substances, along with dead or damaged sperm. The processing also concentrates all viable sperm into an amount ideally suited for Intra Uterine Insemination (IUI), a process by which a physician clinically places sperm within the uterus directly through the cervix.
|Semen deposited inside the vagina||Sperm deposited inside the uterus|
|Cervical mucus is a barrier||Cervical mucus barrier bypassed|
|Cervical mucus prevents entry of seminal plasma||Sperm washing removes seminal plasma|
|Prevents abnormal sperm, non-motile sperm and other cellular forms from entry||Sperm processing removes non-motile sperm and most of the abnormal sperm|
|Only few sperm in an ejaculate are able to enter cervical mucus||Almost all of the viable sperm in an ejaculate are deposited inside the uterus|
Optimum time of IUI depends on any one or all of the following: Hormone levels, ultrasound measurement of your spouse’s follicles, urinary ovulation prediction kit response, and the sperm quality.
Intrauterine insemination (IUI) efficacy depends on the timing of insemination and the number of inseminations.
To achieve high pregnancy success double inseminations is recommended:
|Number of Patients||Pregnancy*|
|Standard IUI protocol: Single insemination 36 hours or later post hCG: “After Ovulation”||535||16.8%|
|Double inseminations protocol: First insemination performed 12 to 18 hours post hCG injection and the second insemination 24 hours following the first: “Before and after Ovulation”||469||31.6%|
Based on the results in the table, first insemination performed before and second insemination after ovulation yielded the highest pregnancy outcomes.
Also, reports on data analysis reveal that the fertile window for IUI is just prior to ovulation**
Please Note: In domesticated animals maximum fertility is achieved if insemination is performed before ovulation.
Insemination therefore must occur before ovulation: it appears that the standard IUI protocol of insemination 30 to 36 hours post hCG may be missing the mark.
Should a single-insemination procedure prove more practical, then the insemination should be performed at the earlier recommended time for both ovulation detection methods.
The success of IUI depends heavily on the immediate availability of quality sperm. In the event that you are not able to provide an ejaculate at the appropriate time, the ejaculate could be incubated overnight in special refrigeration media.
If however ejaculate needs to be available at a later date, then the ejaculate could be cryopreserved and used at the time of ovulation.